Put 3 red beans to 1L glass bottle and 900mL DW, then sterilize in autoclave.

Put 1 Ebios tablet and 100mL Paramecia solution.

Filter 100mL of Paramecia solution to remove debris and check if the Paramecia is alive with microscope.

Add filtered Paramecia solution into sterilized media.

Incubate at room temperature. Passed paramecia can be fed after 3days~.

Manual written in Japanese can be found: https://shigen.nig.ac.jp/zebra/documents/ParameciumNBRP_002.pdf