Paramecia feeding

Use correct bottle for your day of week. Use 200mL from the bottle.

In the bottle, cultured paramecia is spread in the middle and debris is on the bottom.

The first step is purification. Prepare 100mL bottle, funnel and gauze. Fold the gauze twice (make 4 layers) and set on the funnel. Pour the media into the bottle through the gauze and filter debris out. Keep filtered solution and see many paramecia is moving around.(A)

The second step is concentration. Prepare a beaker, large funnel, small funnel and paper filter. Set the large funnel on the beaker, then small funnel and paper filter. Large funnel is only for fixing the small funnel on the beaker and I'll fix this inefficient part later.. Pour filtered media prepared the previous step into the beaker through the paper filter on the small funnel. This step takes long time and I wonder if we can use vacuum. After all the media go through the funnel, do again from the part of (A) for another 100mL.

Pick the paper filter out and place on the white large dish. Pour 50mL of breeding-quality water to the dish and shake gently not to break the paper fiber.

Collect water on the dish into 50mL tube and verify if you get enough paramecia.

Feed paramecia for larvae, 1ml per tank (1 disposable dropper for 2 tanks).

Wash equipments after use.

Put 3 red beans to 1L glass bottle and 900mL DW, then sterilize in autoclave.

Put 1 Ebios tablet and 100mL Paramecia solution.

Filter 100mL of Paramecia solution to remove debris and check if the Paramecia is alive with microscope.

Add filtered Paramecia solution into sterilized media.

Incubate at room temperature. Passed paramecia can be fed after 3days~.

Manual written in Japanese can be found: https://shigen.nig.ac.jp/zebra/documents/ParameciumNBRP_002.pdf