全体概要図

1. FileReaderが非同期的にファイルを読み込むため、http://gakki-uec15.hatenablog.com/entry/2019/09/19/234833を参考に、ファイル読み込みを同期的に行うようにした。
2. email入力欄を作成、入力した項目はサーバに送信

index.pug

.container
  h1 #{title}
  p this is #{title}
  p Upload your fasta file below
  form(action="./upload" method="POST" enctype="multipart/form-data")
    input(type="file" name="file" id="inputfile")
    input(type="text" id="email")
    button(type="button" onclick="file_upload()" value="Upload")
  script.
      var slice_size = 100 * 1024 * 1024;
      var files;
      var file;
      var slice_file;
      var reader = new FileReader();
      var content;
      var sendData = {};
      function file_upload() {
          var slice_file = $("#inputfile")[0].files[0].slice(0,slice_size);
          reader.readAsText(slice_file);
          reader.onload = function() {
              content = reader.result;
              console.log(content);
              sendData.fileContent = content;
              sendData.email = $("#email")[0].value;
              $.ajax({
                  type: "POST",
                  url: "./upload/",
                  data: sendData,
                  dataType: "json",
              })
              .done(function(res){
                  console.log("success");
              })
              .fail(function(res){
                  console.log("falied");
              });
          }
      }

Study

curl https://www.ncbi.nlm.nih.gov/sra/?term=DRR000002   |grep "Study" |awk '{print substr($0, index($0, "Study:"))}'|awk '{print substr($0, 1, index($0, "<div")-1)}' | sed -e 's/Study: <span>//'

Sample

curl https://www.ncbi.nlm.nih.gov/sra/?term=DRR000089  |grep "Sample" |awk '{print substr($0, index($0, "Sample:"))}' |awk '{print substr($0, 1, index($0, "<div")-1)}'| sed -e 's/Sample: <span>//'