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--- daily_maintenance [2023/07/14 05:45] – 133.11.144.12
+++ daily_maintenance [Unknown date] (現在) – 削除 - 外部編集 (Unknown date) 127.0.0.1
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-## Daily maintenance (ver. 23/07/14)
-#### Changelog
-/12/25 Protocol for paramecia was added.
-/01/24 Small modification
-/07/14 New(Paperless check sheet, Jellyfish feeding), Update(protocol for artemia, Protocol for paramecia)
-## Daily check sheet
-Switching from previous paper forms to e-forms as the biotron is too humid and the paper becomes soggy.
-There are QR codes in biotron, scan or [[current_biotron_info|click here]] to access.
-## Check all fish and remove dead fish
-Just check the tanks.
-## Feed fish
-- For the morning part, feed powder food to them. Crushed flake feed in 50 ml centrifuge tube <del>** Large chunk(purple) for adult zebrafish, large chunk(yellow) for adult medaka and small chunk(yellow) for zebrafish and medaka larvae.**</del>
-- For the afternoon part, feed Artemia for adult and paramecia for larvae, respectively. See the way to feed below.
-#### Artemia
-. Shake and mix the stock bottle prepared at least 2 days ago.
-. Filter huge particles including live/dead Artemia, hatched eggshells, and unhatched eggs using fine mesh (usually we use the meshed cage to keep larvae).
-. Wash filtered particles with breeding-quality water (Cl-) to wash the salt out.
-. Collect washed particles in the 50mL tube and incubate for some minutes.
-. Since unhatched eggs quickly sink and hatched eggshells don't sink, the incubated tube will show 3 layers; brown unhatched eggs on the bottom, moving live Artemia above the unhatched egg layer, and hatched eggshells on the top of the tube (See the image below).
-. Collect live Artemia with a plastic pipette carefully and check if live Artemia is successfully collected. Then feed it to fish.
-{{:fa75fbdb-8068-4aed-b846-21f0989fe8b2_1_105_c.jpeg?200|}}
-####Paramecia
-<del>1. Use the correct bottle for your day of the week. Use 200mL from the bottle.
-. In the bottle, cultured paramecia is spread in the middle, and debris is on the bottom.
-. The first step is purification. Prepare an empty 100mL bottle, funnel and gauze. Fold the gauze twice (make 4 layers) and set it on the funnel. Pour the media into the bottle through the gauze and filter debris out. **Keep filtered solution and see many paramecia is moving around.**(A)
-. The second step is concentration. Prepare a beaker, large funnel, small funnel and paper filter. Set the large funnel on the beaker, then a small funnel and paper filter (The large funnel is only for fixing the small funnel on the beaker). Pour filtered media into the beaker through the paper filter on the small funnel. **Do these process twice so that we can prepare 200mL of Paramecia in total.**
-. After all media go through the filter, pick the paper filter out and place it on the white plastic tray. Pour 50mL of breeding-quality water into the tray and shake gently not to break the paper fiber.
-. Collect water from the tray into 50mL tube and verify if you get enough paramecia.
-. Feed paramecia for larvae, 1ml per tank (1 disposable dropper for 2 tanks).
-. Wash all equipment after use.</del>
-Currently, this step is not conducted. We keep larvae in 258.
-## Prepare Artemia stock (For the afternoon part)
-. Wash the bottle (you prepare stock for the bottle which you used on the day)
-. Put 1.5 cups of dried Artemia, 9 cups of salt and 1.5L breeding-quality water into the bottle.
-. Mix the bottle and put an air stone into it. **Check the bubbling! Without sufficient air supply, they don't hatch and all die.**
-## Cleaning
-Clean the water outlet of each tank with a brush. Without this process, tanks become full and fish can come out from the top of the tank. **This causes a possibility of fish strain contamination!**
-## Washing filter
-Wash the mesh filter on the inlet of big tank under the shelf. If they are too dirty, change with new one (cut the stock filter for the appropriate size).
-## Make sure the water tap(above the lower tank) is closed
-Water in the lower big tank immediately evaporates, so make it always full. <del>In this case, you can use the water tap above the tank but **make sure it is closed after use.**</del>
-**We had trouble with the biotron staff several times because we sometimes forget to close the water tap and the main tank (maintained by the other lab!) is dried up. Therefore, DO NOT use the water tap anymore and fill the lower tank manually using the blue bucket**
-## Fill the count list
-<del>Count the number of fish in the specific tank (noted on the daily checksheet)
-</del>
-Currently, this step is not conducted.
-## Medaka feeding on weekend and holiday
-Follow Ogawa-san's instruction, scan the QR code for taking note.
-## Jellyfish feeding in the right-side room
-. Remove the air tube from the incubation bottle.
-. Use a dropper to draw artemia (about 1 mL for each tank) from the middle layer of the incubation bottle.
-. Return the air tube to the incubation bottle.
-. Dilute with equal amount of seawater.
-. One dropper (about 2 mL) for each tank. If the concentration of artemia is low, add a little more appropriately.
-## Jellyfish water changing in the right-side room
-on Tues., Fri. and Sun.
-Follow Shimomura-san's instruction
-## Jellyfish feeding in the left-side room
-. Concentrate artmia in the left side room (when you feed fish, keep some for jellyfish, use sea water to wash)
-. Scoop up some sea water into the tanks
-. Scoop us jellyfish into the tanks(do not mix the different jellyfish)
-. Add artimia to the tanks
-. Put Jellyfish back into original tank after 30 mins.
-. Add some fresh sea water to the original tank