差分
このページの2つのバージョン間の差分を表示します。
| 両方とも前のリビジョン 前のリビジョン 次のリビジョン | 前のリビジョン | ||
| daily_maintenance [2019/12/25 09:00] – 157.82.70.85 | daily_maintenance [Unknown date] (現在) – 削除 - 外部編集 (Unknown date) 127.0.0.1 | ||
|---|---|---|---|
| 行 1: | 行 1: | ||
| - | ## Daily maintenance (ver. 19/12/25) | ||
| - | |||
| - | ## Check all fish and remove dead fish | ||
| - | |||
| - | Just check the tanks. | ||
| - | |||
| - | ## Feed fish | ||
| - | |||
| - | - For the morning part, feed powder food to them.** Large chunk(purple) for adult zebrafish, large chunk(yellow) for adult medaka and small chunk(yellow) for zebrafish and medaka larvae.** | ||
| - | |||
| - | - For the afternoon part, feed Artemia for adult and paramecia for larvae, respectively. See the way to feed below. | ||
| - | |||
| - | #### Artemia | ||
| - | |||
| - | 1. Shake and mix the stock bottle prepared at least 2 days ago. | ||
| - | |||
| - | 1. Filter huge particles including live/dead Artemia, hatched egg shells and unhatched eggs using fine mesh (usually we use meshed cage to keep larvae). | ||
| - | |||
| - | 1. Wash filtered particles with breeding-quality water (Cl-) to wash salt out. | ||
| - | |||
| - | 1. Collect washed particle in the 50mL tube and incubate for some minutes. | ||
| - | |||
| - | 1. Since unhatched eggs quickly sink and hatched egg shells don't sink, incubated tube will show 3 layers; brown unhatched eggs on the bottom, moving live Arteimia above the unhatched egg layer and hatched egg shells on the top of the tube. | ||
| - | |||
| - | 1. Collect live Artemia with plastic pipette carefully and check if live Artemia is successfully collected. Then feed it to fish. | ||
| - | |||
| - | #### | ||
| - | |||
| - | 1. Use correct bottle for your day of week. Use 200mL from the bottle. | ||
| - | |||
| - | 1. In the bottle, cultured paramecia is spread in the middle and debris is on the bottom. | ||
| - | |||
| - | 1. The first step is purification. Prepare 100mL bottle, funnel and gauze. Fold the gauze twice (make 4 layer) and set on the bottle. Pour the media into the bottle through the gauze and filter debris out. **Keep filtered solution and see many paramecia is moving around.**(*) | ||
| - | |||
| - | 1. The second step is concentration. Prepare a beaker, large funnel, small funnel and paper filter. Set the large funnel on the beaker, then small funnel and paper filter. Large funnel is only for fixing the small funnel on the beaker and I'll fix this inefficient part later.. Pour filtered media prepared the previous step into the beaker through the paper filter on the small funnel. This step takes long time and I wonder if we can use vacuum. **After all the media go through the funnel, do again from the part of (*) for another 100mL.** | ||
| - | |||
| - | 1. Pick the paper filter out and place on the white large dish. Pour 50mL of breeding-quality water to the dish and shake gently not to break the paper fiber. | ||
| - | |||
| - | 1. Collect water on the dish into 50mL tube and verify if you get enough paramecia. | ||
| - | |||
| - | 1. Feed paramecia for larvae, 1ml per tank (1 disposable dropper for 2 tanks). | ||
| - | |||
| - | 1. Wash equipments after use. | ||
| - | |||
| - | ## Prepare Artemia stock (For the afternoon part) | ||
| - | |||
| - | 1. Wash the bottle (you prepare stock for the bottle which you used at the day) | ||
| - | |||
| - | 1. Put a half cup of dried Artemia, 3 cups of salt and 500mL breeding-quality water. | ||
| - | |||
| - | 1. Mix and put an air stone into the bottle. **Check the bubbling! Without enough air supply, they don't hatch and all die.** | ||
| - | |||
| - | |||
| - | ## Cleaning | ||
| - | |||
| - | Clean the water outlet of each tank with a brush. Without this process, tanks become full and fish can come out from the top of the tank. **This causes a possibility of fish strain contamination!** | ||
| - | |||
| - | ## Washing filter | ||
| - | |||
| - | Wash the mesh filter on the inlet of big tank under the shelf. If they are too dirty, change with new one (cut the stock filter for the appropriate size). | ||
| - | |||
| - | ## Make sure the water tap(above the lower tank) is closed | ||
| - | |||
| - | Water in the lower big tank immediately evaporate, so make it always full. In this case, you can use the water tap above the tank but **make sure it is closed after use.** | ||
| - | |||
| - | ## Fill the count list | ||
| - | |||
| - | Count the number of fish in the specific tank (noted on the daily checksheet) | ||
| - | |||